Molecular Detection of Ancylostoma duodenale, Ancylostoma ceylanicum, and Necator americanus in Humans in Northeastern and Southern Thailand

The 2 principal species of hookworms infecting humans are Necator americanus and Ancylostoma duodenale. Case studies on zoonotic hookworm infections with Ancylostoma ceylanicum and/or Ancylostoma caninum are known mainly from Asian countries. Of these 2 zoonotic species, only A. ceylanicum can develop to adulthood in humans. In the present study, we report a molecular-based survey of human hookworm infections present in southern and northeastern Thailand. Thirty larval hookworm samples were obtained from fecal agar plate cultures of 10 patients in northeastren Thailand and 20 in southern Thailand. Partial ITS1, 5.8S, and ITS2 regions of the ribosomal DNA genes were amplified using PCR. The amplicons were sequenced, aligned, and compared with other hookworm sequences in GenBank database. The results showed that, in Thailand, N. americanus is more prevalent than Ancylostoma spp. and is found in both study areas. Sporadic cases of A. ceylanicum and A. duodenale infection were seen in northeastern Thailand.

Hallazgo de ancylostoma duodenale en la localidad de Pororó, Paraguay

a) Se realizó un trabajo de prevalencia de parásitosintestinales, en la localidad de Pororó. Se encotro que el 93 porciento de la población estudiada estabaparasitada, 61 prciento poseían dos o más parásitos.Entre los principales parásitos intestinales encontrados están: blastocystis hominis 71,3 porciento, uncinaria 52,8 poriento y giardialamblia 19,7 porcientoTambién se realizó el cultivo de las heces por método de Harada-Mori, se encontro: necator americanusen el 68porciento de las muestras, ancylostomaduodenaleen el 36 porciento y strongyloides stercoralis en el4 porciento las cifras de ancylostoma duodenale son superior